List/Grid CRISPR Technology

Screen Shot 2015-01-02 at 9.38.31 PM

Guide-Seq: Genome-wide off-target cleavage sites of CRISPR-Cas nucleases

Massachusetts General Hospital (MGH) investigators have developed a method of detecting, across the entire genome of human
Screen Shot 2015-01-02 at 9.47.07 PM

New way to turn genes on using CRISPR-Cas9

Using a gene-editing system originally developed to delete specific genes, MIT researchers have now shown that they can
This image shows fluorescence visualization of a gill in a frog embryo using the PITCh knock-in system. The arrow indicates the gill (green). Image Credit:
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University

A novel technique for gene insertion by genome editing

Using a novel gene knock-in technique, effective insertion of an exogenous gene was demonstrated in human cells and in animal Incoming search terms:Crispr gene insertion

New genome-editing platform significantly increases accuracy of CRISPR-based systems

A next-generation genome editing system developed by Massachusetts General Hospital (MGH) investigators substantially decreases the risk of
NSF's 2014 Alan T. Waterman Awardee Feng Zhang is an investigator at the McGovern Institute for Brain Research at MIT and a core member of the Broad Institute.

Credit: Kent Dayton

Feng Zhang wins Alan T. Waterman Award for his revolutionary CRISPR work

The National Science Foundation (NSF) named Feng Zhang the 2014 recipient of its Alan T. Waterman Award.
steptococcus pyogens

The many faces of the bacterial defense system

Even bacteria have a kind of “immune system” they use to defend themselves against unwanted intruders – in their case,
This image of the Cas9 complex depicts the Cas9 protein (in light 
blue), along with its guide RNA (yellow), and target DNA (red). 
Image courtesy of Bang Wong, from source material provided by 
Feng Zhang.

Broad Institute gets first patent on revolutionary CRISPR-Cas9 system

The Broad Institute today announced that the United States Patent and Trademark Office has issued the first patent for an
crispr peptide

Cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA

RNA-guided endonucleases (RGENs) derived from the CRISPR/Cas system represent an efficient tool for genome editing. RGENs consist of two components: Cas9 protein and guide RNA. Plasmid-mediated ...
This image of the Cas9 complex depicts the Cas9 protein (in light 
blue), along with its guide RNA (yellow), and target DNA (red). 
Image courtesy of Bang Wong, from source material provided by 
Feng Zhang.

Researchers reveal structure of key CRISPR complex

Researchers from the Broad Institute and MIT have teamed up with colleagues from the University of Tokyo to form the first high definition
The crystal structure of SpyCas9 features a nuclease domain lobe (red) and an alpha-helical lobe (gray) each with a nucleic acid binding cleft that becomes functionalized when Cas9 binds to guide RNA.

Credit: Image courtesy of Doudna, Nogales, et. al

Researchers show expanded role for guide RNA in Cas9 interactions with DNA

The potential is there for bacteria and other microbes to be genetically engineered to perform a cornucopia of valuable
© 2017 CRISPR tools and Resources. All rights reserved.