List/Grid CRISPR Specificity

RGEN-induced ‘digenome’ sequencing to capture off-target sites. (a) Overview of Digenome-seq. Forward and reverse sequence reads are shown in pink and blue, respectively. Red triangles and vertical lines indicate cleavage positions. WT, wild type.

End of CRISPR-Cas9 controversy

Researchers hahave successfully confirmed that CRISPR-Cas9 has accurate on-target effects in human cells. There has been great interest in CRISPR-Cas9 as a tool to develop anticancer ...
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