Latest Updates on CRISPR-cas Technology

nicotiana-tabacum

CRISPR/Cas9-mediated targeted mutagenesis in Nicotiana tabacum.

Genome editing is one of the most powerful tools for revealing gene function and improving crop plants. Recently, RNA-guided genome editing using the

New genome-editing platform significantly increases accuracy of CRISPR-based systems

A next-generation genome editing system developed by Massachusetts General Hospital (MGH) investigators substantially decreases the risk of
NSF's 2014 Alan T. Waterman Awardee Feng Zhang is an investigator at the McGovern Institute for Brain Research at MIT and a core member of the Broad Institute.

Credit: Kent Dayton

Feng Zhang wins Alan T. Waterman Award for his revolutionary CRISPR work

The National Science Foundation (NSF) named Feng Zhang the 2014 recipient of its Alan T. Waterman Award.
steptococcus pyogens

The many faces of the bacterial defense system

Even bacteria have a kind of “immune system” they use to defend themselves against unwanted intruders – in their case,
This image of the Cas9 complex depicts the Cas9 protein (in light 
blue), along with its guide RNA (yellow), and target DNA (red). 
Image courtesy of Bang Wong, from source material provided by 
Feng Zhang.

Broad Institute gets first patent on revolutionary CRISPR-Cas9 system

The Broad Institute today announced that the United States Patent and Trademark Office has issued the first patent for an
crispr peptide

Cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA

RNA-guided endonucleases (RGENs) derived from the CRISPR/Cas system represent an efficient tool for genome editing. RGENs consist of two components: Cas9 protein and guide RNA. Plasmid-mediated ...
Image Credit: Christine Daniloff/MIT

Researchers cured mice of a rare liver disorder caused by a single genetic mutation using CRISPR-cas9

Using a new gene-editing system based on bacterial proteins, MIT researchers have cured mice of a rare liver disorder caused by a single genetic mutation. 
This image of the Cas9 complex depicts the Cas9 protein (in light 
blue), along with its guide RNA (yellow), and target DNA (red). 
Image courtesy of Bang Wong, from source material provided by 
Feng Zhang.

Researchers reveal structure of key CRISPR complex

Researchers from the Broad Institute and MIT have teamed up with colleagues from the University of Tokyo to form the first high definition
CRISPRCasOutput1

ZiFiT Targeter tool for designing CRISPR, CRISPR nickase targets

ZiFiT was originally developed by the Keith Joung lab  as a tool for the identification of potential zinc finger nulcease (ZFN) sites in specific target sequences. This tool is now ...
The crystal structure of SpyCas9 features a nuclease domain lobe (red) and an alpha-helical lobe (gray) each with a nucleic acid binding cleft that becomes functionalized when Cas9 binds to guide RNA.

Credit: Image courtesy of Doudna, Nogales, et. al

Researchers show expanded role for guide RNA in Cas9 interactions with DNA

The potential is there for bacteria and other microbes to be genetically engineered to perform a cornucopia of valuable
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