List/Grid Tag Archives: featured

crispr small molecule

CRISPR-mediated gene editing using small molecules

One of the most exciting scientific advances made in recent years is CRISPR—the ability to precisely edit the genome of cells.
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Guide-Seq: Genome-wide off-target cleavage sites of CRISPR-Cas nucleases

Massachusetts General Hospital (MGH) investigators have developed a method of detecting, across the entire genome of human
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New way to turn genes on using CRISPR-Cas9

Using a gene-editing system originally developed to delete specific genes, MIT researchers have now shown that they can
steptococcus pyogens

The many faces of the bacterial defense system

Even bacteria have a kind of “immune system” they use to defend themselves against unwanted intruders – in their case,
This image of the Cas9 complex depicts the Cas9 protein (in light 
blue), along with its guide RNA (yellow), and target DNA (red). 
Image courtesy of Bang Wong, from source material provided by 
Feng Zhang.

Broad Institute gets first patent on revolutionary CRISPR-Cas9 system

The Broad Institute today announced that the United States Patent and Trademark Office has issued the first patent for an
This image of the Cas9 complex depicts the Cas9 protein (in light 
blue), along with its guide RNA (yellow), and target DNA (red). 
Image courtesy of Bang Wong, from source material provided by 
Feng Zhang.

Researchers reveal structure of key CRISPR complex

Researchers from the Broad Institute and MIT have teamed up with colleagues from the University of Tokyo to form the first high definition
The crystal structure of SpyCas9 features a nuclease domain lobe (red) and an alpha-helical lobe (gray) each with a nucleic acid binding cleft that becomes functionalized when Cas9 binds to guide RNA.

Credit: Image courtesy of Doudna, Nogales, et. al

Researchers show expanded role for guide RNA in Cas9 interactions with DNA

The potential is there for bacteria and other microbes to be genetically engineered to perform a cornucopia of valuable
Jennifer Doudna and Samuel Sternberg used a combination of single-molecule imaging and bulk biochemical experiments to show how the RNA-guided Cas9 enzyme is able to locate specific 20-base-pair target sequences within genomes that are millions to billions of base pairs long. (Photo by Roy Kaltschmdit)

Mystery of how Cas9 Targets and Cleaves DNA solved.

A central question has been answered regarding a protein that plays an essential role in the bacterial immune system and is fast becoming a
Credit: Cell, Niu et al.

Researchers achieved precise gene modification in monkeys using CRISPR-cas9

Monkeys are important for modeling diseases because of their close similarities to humans, but past efforts to precisely modify genes in
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